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Preheat oven to 375 degrees F.
Place the pie crust on a 9-inch pie plate and pre-bake for 10 minutes.
In a large skillet, melt the butter over medium heat.
Add the onion and garlic and cook until the onion is translucent about 5 minutes.
Add the spinach and cook until wilted, about 2 minutes.
Add the tuna and cook for 2 minutes.
In a medium bowl, whisk together the eggs, milk, cheese, salt, and pepper.
Add the cooked vegetables to the egg mixture and stir to combine.
Pour the mixture into the pre-baked crust and bake for 25-30 minutes, until the centre is set.
Let cool before serving.